Minimum sequencing depth when using single individual to infer demographic.

[xiekunwhy]

Hi,

If there is only one individual, how depth I need to sequence (whole genome sequencing) if I want to get reliability results using magic, 5X, 10X, 20X or 30X ?

Best,
Kun

[weissman]

Probably 30X. You want at least 80-90% of the sites to have high-quality calls, and you don't want there to be big biases in what gets called.

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On Fri, Mar 5, 2021 at 1:39 AM xiekunwhy ***@***.***> wrote: Hi, If there is only one individual, how depth I need to sequence (whole genome sequencing) if I want to get reliability results using magic, 5X, 10X, 20X or 30X ? Best, Kun — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#7>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ACDUUQF3PZHLAH7XUC2YQ2TTCB4CTANCNFSM4YUUVPEQ> .

[xiekunwhy]

I see, thank you for your answers.