diff --git a/CHANGES/11085.misc.rst b/CHANGES/11085.misc.rst
new file mode 100644
index 00000000000..67b1915cfcb
--- /dev/null
+++ b/CHANGES/11085.misc.rst
@@ -0,0 +1 @@
+Improved performance of isinstance checks by using collections.abc types instead of typing module equivalents -- by :user:`bdraco`.
diff --git a/aiohttp/client_reqrep.py b/aiohttp/client_reqrep.py
index 972c32969fd..de62f94da84 100644
--- a/aiohttp/client_reqrep.py
+++ b/aiohttp/client_reqrep.py
@@ -7,6 +7,7 @@
 import sys
 import traceback
 import warnings
+from collections.abc import Mapping as ABCMapping
 from hashlib import md5, sha1, sha256
 from http.cookies import CookieError, Morsel, SimpleCookie
 from types import MappingProxyType, TracebackType
@@ -18,7 +19,6 @@
     Iterable,
     List,
     Literal,
-    Mapping,
     NamedTuple,
     Optional,
     Tuple,
@@ -1006,7 +1006,7 @@ def update_cookies(self, cookies: Optional[LooseCookies]) -> None:
             c.load(self.headers.get(hdrs.COOKIE, ""))
             del self.headers[hdrs.COOKIE]
 
-        if isinstance(cookies, Mapping):
+        if isinstance(cookies, ABCMapping):
             iter_cookies = cookies.items()
         else:
             iter_cookies = cookies  # type: ignore[assignment]
diff --git a/aiohttp/cookiejar.py b/aiohttp/cookiejar.py
index 1e286dffed2..d337944751f 100644
--- a/aiohttp/cookiejar.py
+++ b/aiohttp/cookiejar.py
@@ -10,6 +10,7 @@
 import time
 import warnings
 from collections import defaultdict
+from collections.abc import Mapping as ABCMapping
 from http.cookies import BaseCookie, Morsel, SimpleCookie
 from typing import (
     DefaultDict,
@@ -18,7 +19,6 @@
     Iterable,
     Iterator,
     List,
-    Mapping,
     Optional,
     Set,
     Tuple,
@@ -237,7 +237,7 @@ def update_cookies(self, cookies: LooseCookies, response_url: URL = URL()) -> No
             # Don't accept cookies from IPs
             return
 
-        if isinstance(cookies, Mapping):
+        if isinstance(cookies, ABCMapping):
             cookies = cookies.items()
 
         for name, cookie in cookies:
diff --git a/aiohttp/multipart.py b/aiohttp/multipart.py
index fe5499e1144..c32eb4eb122 100644
--- a/aiohttp/multipart.py
+++ b/aiohttp/multipart.py
@@ -6,6 +6,7 @@
 import uuid
 import warnings
 from collections import deque
+from collections.abc import Mapping as ABCMapping, Sequence as ABCSequence
 from types import TracebackType
 from typing import (
     TYPE_CHECKING,
@@ -957,12 +958,12 @@ def append_form(
         headers: Optional[Mapping[str, str]] = None,
     ) -> Payload:
         """Helper to append form urlencoded part."""
-        assert isinstance(obj, (Sequence, Mapping))
+        assert isinstance(obj, (ABCSequence, ABCMapping))
 
         if headers is None:
             headers = CIMultiDict()
 
-        if isinstance(obj, Mapping):
+        if isinstance(obj, ABCMapping):
             obj = list(obj.items())
         data = urlencode(obj, doseq=True)
 
diff --git a/aiohttp/payload.py b/aiohttp/payload.py
index dbe12ed639c..de12c758157 100644
--- a/aiohttp/payload.py
+++ b/aiohttp/payload.py
@@ -7,6 +7,7 @@
 import sys
 import warnings
 from abc import ABC, abstractmethod
+from collections.abc import Iterable as ABCIterable
 from itertools import chain
 from typing import (
     IO,
@@ -137,7 +138,7 @@ def register(
             self._first.append((factory, type))
         elif order is Order.normal:
             self._normal.append((factory, type))
-            if isinstance(type, Iterable):
+            if isinstance(type, ABCIterable):
                 for t in type:
                     self._normal_lookup[t] = factory
             else: