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Hello, I'm trying to use the R script for CTF_normalization for my work and some of my data has missing values or NAs. How to deal with NAs I'm getting an error,
norm_factors <- calcNormFactors(object = t, lib.size = lib_size, method = "TMM")
Error in calcNormFactors.default(object = t, lib.size = lib_size, method = "TMM") :
NA counts not permitted
Please suggest how should I proceed. Thanks.
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