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Hi, I encountered a warning and an error when loading an AnnData object using anndataToGiotto():
> giotto.obj <- anndataToGiotto(data_path)
...
Warning: Key 'connectivities' not found in adata.uns.
Error in py_call_impl(callable, call_args$unnamed, call_args$named) :
TypeError: 'NoneType' object is not subscriptable
Run `reticulate::py_last_error()` for details.
> reticulate::py_last_error()
── Python Exception Message ────────────────────────────────────────────────────────────────────────────────────────────────────
Traceback (most recent call last):
File "/Library/Frameworks/R.framework/Versions/4.4-x86_64/Resources/library/GiottoClass/python/ad2g.py", line 322, in align_network_data
w_nz = weights[idx_dist_not_sparse].astype(float)
TypeError: 'NoneType' object is not subscriptable
── R Traceback ─────────────────────────────────────────────────────────────────────────────────────────────────────────────────
▆
1. └─GiottoClass::anndataToGiotto(data_path)
2. └─reticulate (local) align_network_data(distances = weights_ad, weights = distances_ad)
3. └─reticulate:::py_call_impl(callable, call_args$unnamed, call_args$named)
See `reticulate::py_last_error()$r_trace$full_call` for more details.
The AnnData object was first converted from a Xenium dataset using the spatialdata Python package and then processed using Scanpy (but not Giotto). Since the error seems to be related to nearest neighbor networks, I am also listing relevant info below:
> adata.uns['neighbors']
'connectivities_key': 'connectivities',
'distances_key': 'distances',
'params': {'method': 'umap',
'metric': 'euclidean',
'n_neighbors': 15,
'n_pcs': 20,
'random_state': 0}}
> adata.obsp
PairwiseArrays with keys: connectivities, distances
> adata.obsp['connectivities']
<Compressed Sparse Row sparse matrix of dtype 'float32'
with 20864442 stored elements and shape (960632, 960632)>
> adata.obsp['distances']
<Compressed Sparse Row sparse matrix of dtype 'float32'
with 15370112 stored elements and shape (960632, 960632)>
Software versions:
- R: 4.4.3
- Giotto: 4.2.2
- GiottoClass: 0.4.9
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