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Description
Hi,
When I run "../tools/bin/bpipe run -p EXOME_TARGET="SCA8_region.bed" ../pipelines/STRetch_exome_pipeline.groovy *.fastq.gz" for testing, it return such errors:
File "/opt/STRetch/tools/miniconda/envs/STR/lib/python3.7/site-packages/Bio/Alphabet/init.py", line 21, in
"Bio.Alphabet has been removed from Biopython. In many cases, the alphabet can simply be ignored and removed from scripts. In a few cases, you may need to specify the molecule_type as an annotation on a SeqRecord for your script to work correctly. Please see https://biopython.org/wiki/Alphabet for more information."
ImportError: Bio.Alphabet has been removed from Biopython. In many cases, the alphabet can simply be ignored and removed from scripts. In a few cases, you may need to specify the molecule_type as an annotation on a SeqRecord for your script to work correctly. Please see https://biopython.org/wiki/Alphabet for more information.
ERROR: stage STR_locus_counts failed: Command in stage STR_locus_counts failed with exit status = 1 :
......
STRPATH=$PATH; PATH=/opt/STRetch/tools/bin:$PATH; /opt/STRetch/tools/bin/python /opt/STRetch/scripts/identify_locus.py --bam 11_L001_R1.STRdecoy.bam --bed /opt/STRetch/reference-data/hg19.simpleRepeat_period1-6_dedup.sorted.bed --output 11_L001_R1.STRdecoy.locus_counts ;PATH=$STRPATH
ERROR: stage STR_locus_counts failed: Command in stage STR_locus_counts failed with exit status = 1 :