From 0a24ce714f4a6f46a463d05b49ba4eafcad6a9dd Mon Sep 17 00:00:00 2001 From: EnfxcFCb6 Date: Mon, 2 Jun 2025 12:41:22 -0400 Subject: [PATCH 1/2] Standardized README to Markdown format --- README.md | 35 +++++++++++++++++++++++++++++++ readme.html | 59 ----------------------------------------------------- 2 files changed, 35 insertions(+), 59 deletions(-) create mode 100644 README.md delete mode 100644 readme.html diff --git a/README.md b/README.md new file mode 100644 index 0000000..8a9b1ad --- /dev/null +++ b/README.md @@ -0,0 +1,35 @@ +This is a readme file for the models associated with the paper: + +Yen-Ching Liu et al., The mechanisms of propofol-induced block on ion +currents in differentiated H9c2 cardiac cells. +*Eur. J. Pharmacol.* 2008;590: 93-98. + +### Abstract + +General anesthetic propofol (2,6-bis(isopropyl)-phenol) possess a chemical structure unrelated to other anesthetic drugs. It has been known to block a variety of ion currents. This study is designed to determine the effect of this drug on ion currents in differentiated H9c2 cardiac cells. The effects of propofol, an intravenous anesthetic agent with a distinct chemical structure, on ion currents of differentiated clonal cardiac (H9c2) cells were investigated in this study. Propofol (10-300 μM) suppressed the amplitude of delayed rectifier K(+) current (I(K(DR))) in a concentration-dependent manner with an IC(50) value of 36 μM. This compound reduced activation time constant and increased current inactivation, although no voltage dependency of propofol-induced block of I(K(DR)) can demonstrated. Neither diazoxide, pinacidil, nor caffeic acid phenethyl ester had any effect on propofol-induced block of I(K(DR)). Propofol (30 μM) had no effect on erg-mediated K(+) current in these cells; however, it suppressed L-type Ca(2+) current (I(Ca,L)) of cardiac and skeletal types to a similar extent. Intracellular dialysis with propofol (100 μM) had no effects on I(K(DR)) or I(Ca,L). Numerical simulations of I(K(DR)) based on a Markovian model reproduce the experimental results and show that propofol-induced blockade of I(K(DR)) is associated with an decrease in forward rate of the activation process and an increase in transitional rate into the inactivated state. Propofol can suppress I(K(DR)) in differentiated H9c2 cardiac cells in a concentration- and state- dependent manner. These effects can significantly contribute its action on functional activity of heart cells. + +### To run the models: + +**XPP:** start with the command +`xpp IK_Prop.ode` +Mouse click on "Initialconds", and then "(G)o". + +Regarding xpp program, please contact Bard Ermentrout's website: +[http://www.pitt.edu/~phase/](http://www.pitt.edu/~phase/) +Here describes the details in how to get and use xpp. + +To run a series of voltage-clamp studies shown in IK-prop.jpg, click +Initialconds -> Range, then under "Range over", change to 'vtest_1', and then select voltage protocol from Steps (4), Start (10) and End (+50). Change Cycle Color to yes and press (G)o, This makes traces: + +![screenshot](./IK-prop.JPG) + +The model file was supplied by: + +Dr. Sheng-Nan Wu +National Cheng Kung University Medical College +Tainan 70101, Taiwan +snwu@mail.ncku.edu.tw + +--- + +2025-06-02: Standardized to Markdown. \ No newline at end of file diff --git a/readme.html b/readme.html deleted file mode 100644 index d5d9b44..0000000 --- a/readme.html +++ /dev/null @@ -1,59 +0,0 @@ -
-This is a readme file for the models associated with the paper:
-
-Yen-Ching Liu et al., The mechanisms of propofol-induced block on ion
-currents in differentiated H9c2 cardiac cells.
-Eur. J. Pharmacol. 2008;590: 93-98.
-
-Abstract: General anesthetic propofol (2,6-bis(isopropyl)-phenol)
-possess a chemical structure unrelated to other anesthetic drugs. It
-has been known to block a variety of ion currents. This study is
-designed to determine the effect of this drug on ion currents in
-differentiated H9c2 cardiac cells. The effects of propofol, an
-intravenous anesthetic agent with a distinct chemical structure, on
-ion currents of differentiated clonal cardiac (H9c2) cells were
-investigated in this study. Propofol (10-300 muM) suppressed the
-amplitude of delayed rectifier K(+) current (I(K(DR))) in a
-concentration-dependent manner with an IC(50) value of 36 muM. This
-compound reduced activation time constant and increased current
-inactivation, although no voltage dependency of propofol-induced block
-of I(K(DR)) can demonstrated. Neither diazoxide, pinacidil, nor
-caffeic acid phenethyl ester had any effect on propofol-induced block
-of I(K(DR)). Propofol (30 muM) had no effect on erg-mediated K(+)
-current in these cells; however, it suppressed L-type Ca(2+) current
-(I(Ca,L)) of cardiac and skeletal types to a similar
-extent. Intracellular dialysis with propofol (100 muM) had no effects
-on I(K(DR)) or I(Ca,L). Numerical simulations of I(K(DR)) based on a
-Markovian model reproduce the experimental results and show that
-propofol-induced blockade of I(K(DR)) is associated with an decrease
-in forward rate of the activation process and an increase in
-transitional rate into the inactivated state. Propofol can suppress
-I(K(DR)) in differentiated H9c2 cardiac cells in a concentration- and
-state- dependent manner. These effects can significantly contribute
-its action on functional activity of heart cells.
-
-
-To run the models:
-
-XPP: start with the command
-xpp IK_Prop.ode
-Mouse click on "Initialconds", and then "(G)o".
-
-Regarding xpp program, please contact with Bard Ermentrout's website: 
- http://www.pitt.edu/~phase/ 
-Here describes the details in how to get and use xpp.
-
-To run a series of voltage-clamp studies shown in IK-prop.jpg, click
-Initialconds -> Range, then under "Range over", change to 'vtest_1',
-and then select voltage protocol from Steps (4), Start (10) and End
-(+50). Change Cycle Color to yes and press (G)o, This makes traces:
-
-screenshot
-
-The model file was supplied by:
-
-Dr. Sheng-Nan Wu
-National Cheng Kung University Medical College
-Tainan 70101, Taiwan
-snwu@mail.ncku.edu.tw
-
From 45d0b2409ac661bc9914eec01ac4dd1af293ddbb Mon Sep 17 00:00:00 2001 From: rsakai Date: Mon, 2 Jun 2025 13:34:39 -0400 Subject: [PATCH 2/2] Update README.md --- README.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 8a9b1ad..d186574 100644 --- a/README.md +++ b/README.md @@ -25,11 +25,11 @@ Initialconds -> Range, then under "Range over", change to 'vtest_1', and then se The model file was supplied by: -Dr. Sheng-Nan Wu -National Cheng Kung University Medical College -Tainan 70101, Taiwan +Dr. Sheng-Nan Wu +National Cheng Kung University Medical College +Tainan 70101, Taiwan snwu@mail.ncku.edu.tw --- -2025-06-02: Standardized to Markdown. \ No newline at end of file +2025-06-02: Standardized to Markdown.